One who wakes up in the middle of the night before עלות השחר and plans to remain awake until morning, should wash their hands without saying the bracha of al nitilas yadayim, and wash again by עלות השחר with a bracha. Similarly, one who was awake the whole night should wash without a bracha. However, if one used the restroom before davening then they should wash their hands with reciting a bracha.